BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Dissolve the frozen tube in a 28 ℃ water bath and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Growth conditions | 28 ℃, 5-7 days, aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Spread growth, filamentous, irregular edges, opaque, front gray white, rough surface, dry texture, later black sclerotia growth |
| Sharing mode | Public welfare sharing |
Sclerotinia sclerotiorum
Storage conditions: 2~8 ℃
No.: 122299
Product format : agar slant in 14mm test tube
Validity period: 30 days
Biosafety level : 1, handle in ultra-clear table or safety cabinet
Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :25-28 ℃, aerobic, integrated PDA,4d. Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability, in 5 days plate colony is obvious |
| colony morphology: |
Small filamentous fungi, with obvious colonies on integrated PDA medium, turning gray after initial white, gray inside, dense and low flat, Spread and grow to concentric circles on the edge of the plate, and the back of the medium is dark brown. |
| Conclusion: | good viability, no abnormal colony morphology, qualified |
Standard quality control samples of heat-resistant coliform bacteria in drinking water (multi-tube fermentation method)
BNCC360414
Quantitative strains of aspergillus niger
BNCC353785
Quantitative strains of candida albicans
BNCC373402
8032 Staphylococcus albus infection carrier (cloth)
BNCC362004
Quantitative strains of bacillus subtilis
BNCC376273
Quantitative strains of Micrococcus sp.
BNCC372974
- English
- Japanese
