Rooting rhizobia|133856 |BNCC

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Rhizobium rhizogenes

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28-30 ℃, 24-48 hours, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2 mm，Shape: Circular，Margin: Regular，Transparency: Opaque，Color: Pink，Elevation: Raised center，Surface: Glossy and smooth，Texture: Moist and easy to lift
Sharing mode	Public welfare sharing

Rhizobium rhizogenes

Storage conditions : 2~8 ℃

No. : 133856

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, rhizobia medium, 24-48h. Rhizobium culture medium: KH₂PO₄ 0.25g,K₂HPO₄ 0.25g,MgSO₄·7H₂O 0.2 g,NaCl 0.1g, yeast extract 0.8g, mannitol 10.0g, agar 18.0g (not included in liquid medium), distilled water 1.0 L,pH 7.2. 121 ℃,15min sterilization

activation steps :

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2)Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation,and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 24-48h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology (above)	size: 0.5-1mm shape: round edge: irregular transparency: opaque Color: off-white uplift: middle raised surface: smooth texture: wet
Conclusion	good viability, no abnormal colony morphology, qualified

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