Bacillus subtilis|195654 |BNCC

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Bacillus subtilis

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Test the liquid
Growth conditions	37 ℃, 18-24h, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 2-4mm Shape: Irregular Edge: Irregular Transparency: Opaque Color: Grey White Rise: Flat Surface: Rough Texture: Dry
Sharing mode	Public welfare sharing

1.Description

1. Name : Bacillus subtilis

2. BNCC No.: 195654

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. Nutritious gravy agar: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4 .H2O when culturing Bacillus,which is beneficial to spore production.

2. Atmosphere: aerobic

3,Temperature: 37 ℃,24h

4. Notes

1. Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3. Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4. Waste generated from the handling process should be discarded after high-pressure sterilization

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