Klebsiella genus|196052 |BNCC

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Klebsiella sp.

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium or sterile water and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	30 ℃, 18-24h, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front and yellow on the back, protruding in the middle, smooth on the surface, moist in texture, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Klebsiella sp.

BNCC No.：196052

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul

Validity period: freeze-dried tube for 24 months

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, LB broth medium. LB broth medium: peptone 10.0g, yeast powder 5.0g,NaCl 10.0g, distilled water 1.0L,pH7.0.

Recovery steps:

(1)Prepare a flask of LB liquid media

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 28℃ for 18-24 hours (140r/min);

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 20h LB bacterial fluid is turbid, LB plate colony is obvious
colony morphology:	size: medium color: orange yellow shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque bulge: middle bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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