Firmicutes|142822 |BNCC

Essential Information Certificate Related Products

Acremonium strictum

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony appears white in the early stages and turns pale pink in the later stages
Sharing mode	Public welfare sharing

Acremonium strictum

Storage conditions : 2~8 ℃

No. : 142822

Product format : agar slant in 14mm test tube

Validity period : 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, glucose 20.0g, vitamin B1 10mg, agar 20g,pH natural. 121 ℃,15min. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

Recovery steps:

(1) Prepare 1-2 pieces of PDA plates

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

(5) Put the plates under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability,in 6 days plate colony is obvious
colony morphology:	small filamentous fungi, colonies are obviously visible on comprehensive PDA medium, hyphae are white, dense and thick.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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