Bacillus licheniformis|221854 |BNCC

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Bacillus lichenformis

Subculture procedure	① Prepare 1-2 fresh plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	30 ℃; 24-48 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, white on the front, flat in shape, rough on the surface, G+(blue purple), rod-shaped, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name：Bacillus lichenformis

2. BNCC No.：221854

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. nutrient gravy medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [note] adding 5mg MnSO₄·H₂O when culturing Bacillus，which is beneficial to spore production.

2. Atmosphere：aerobic

3. Temperature： 30 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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