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Bifidobacterium pseudocatenulatum

Subculture procedure	① Prepare 1-2 fresh tablets mentioned above (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium or sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. Dissolve it thoroughly and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 24-48 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, milky white on the front, raised in the middle, smooth surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Bifidobacterium pseudocatenulatum

Storage conditions: 2~8 ℃

No. 134343

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, anaerobic, BBL medium, 24-48h, BBL medium: peptone 15.0g, glucose 20.0g, yeast extract 2.0g, soluble starch 0.5g, sodium chloride 5.0g, L-cysteine 0.5g , Tomato extract powder 5.0g, liver extract powder 2.0g, Tween 80 1mL, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 7.0±0.1. Sterilize at 121℃ for 15min.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates（place in anaerobic environment for 24hours before usage）;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 24-48h liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology: (above)	size: 05.-1mm ; Shape: round ; Edge: neat ; Transparency: opaque color: milky white ; Protuberance: middle bulge ; Surface: bright and smooth &; Texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified

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