Lingzhi (Purple Ganoderma)|382030 |BNCC

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Ganoderma lucidum

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 7-10 days; aerobic;
Subculture procedure	Prepare one 90mm solid agar plate, one inoculation shovel, and one inoculation hoe for typical bacterial colonies; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment (if there is paraffin, all paraffin should be sucked out), first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Storage conditions	-80 ℃
Safety level	1
morphology	Spread growth, filamentous, irregular edges, opaque, white front, flat shape, rough surface, wrinkled surface, dry texture, purity: pure
Sharing mode	Public welfare sharing