Nitrobacter genus|382385 |BNCC

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Azoarcus sp.

Culture medium	BNCC352246
Description	R2A Agar
Composition	Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K₂HPO₄:0.3g, MgSO₄:0.024g, Sodium Pyruvate(C₃H₃NaO₃):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃)
Growth conditions	28 ℃; 48-72h; aerobic
Subculture procedure	① Dissolve the frozen tube in a 30 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), rod-shaped
Sharing mode	Public welfare sharing