Salmonella saintpaul

Storage conditions: 2~8 ℃

No.: 192087

Product format: freeze dried, 200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Activation/passage:37°C, aerobic, nutrient agar medium (NA). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

①Prepare 1 bottle of NA liquid culture medium (100ml triangular bottle is divided into 50ml culture medium) or 2 NA plates;

②Open the safety tube in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③Suck 0.5mL of liquid culture medium and put it into a freeze-drying tube. after fully dissolving, all the bacteria suspension will be beaten back into the liquid culture medium, mixed evenly, and cultured in a 37 ℃ shaker. Or directly suck the bacterial suspension (200 & mu; L/a) hit NA plate, evenly coated, the plate placed in a 37 ℃ incubator for 18-24h.

Recovery record: According to the activation requirements, the above strains were resuscitated, and the recorded results are as follows: