False honey ring fungus (honey ring fungus, honey ring fungus, hazelnut mushroom)|143145 |BNCC

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Armillaria mellea

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap and transfer the solution and its bacterial block into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi with obvious colonies on PDA medium, slowly spreading, initially white hyphae, later turning black brown, and growing false roots in the medium
Sharing mode	Public welfare sharing

1. Description

1. Name: Armillaria mellea

2. BNCC No.: 143145

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Comprehensive PDA agar: potato extract 1.0L, glucose 20.0g ,KH₂PO₄3.0g ,MgSO₄.7H₂O1.5g, vitamin B1 trace g, agar 15.0g ,pH 6.0

2. Atmosphere: aerobic

3. Temperature: 25 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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