Elegant small gram silver fungi|145924 |BNCC

Essential Information Certificate Related Products

Cunninghamella elegans

Subculture procedure	① Dissolve the Frozen vials in a 30 ℃ water bath and shake them rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi, with clearly visible colonies on comprehensive PDA medium, white colonies, flocculent hyphae, dense cotton, vigorous growth, and rapid spread
Sharing mode	Public welfare sharing

Cunninghamella elegans

Storage conditions: 2~8 ℃

No.: 145924

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, comprehensive PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1) Prepare 1-2 pieces of PDA plates;

(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

(5) Put the plates under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 3 days plate colony is obvious
colony morphology:	small filamentous fungi, colonies are obviously visible on comprehensive PDA culture medium, colonies are white, hyphae are flocculent, cotton is dense, grows vigorously and spreads rapidly.
conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

Download certificate