Clostridium|185375 |BNCC

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Clostridium sp.

Subculture procedure	① Prepare one 18mm test tube liquid culture medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Clostridium beijerinckii is an anaerobic bacterium. Microscopic examination revealed Gram-positive rods. After 48 hours of cultivation in FT medium, a significant amount of sediment was observed at the bottom of the test tube, accompanied by gas production.Growth of the organism is also supported on ready-to-use Columbia blood agar plates under anaerobic conditions.
Sharing mode	Public welfare sharing

Clostridium butyricum

Storage conditions : 2~8 ℃

No. : 185375

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37 ℃, anaerobic, thioglycolate liquid medium, 24-48h. Thioglycolate liquid medium: caseptone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, sodium chloride 2.5g, azure 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare 1 φ18mm test tube liquid culture medium, about 10mL (placed in anaerobic environment for 24 hours in advance);

② disinfect the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning place to break it, and then knock it open with tweezers;

③ suck about 0.5mL of liquid culture medium into lyophilized tube, fully dissolve, suck back into test tube liquid culture medium, and mix well;

④ place the liquid test tube in the above-specified Culture conditions Under culture, the bacterial liquid can be used if it is turbid or there is a large amount of bacterial growth at the bottom. Microscopic examination of bacteria liquid

Recovery record: activation is carried out according to activation requirements, and the recording results are as follows:

Item	test results
viability:	good viability, in 24 hours liquid medium become turbid
resurrection effect and microscopic examination: (above)	Clostridium butyricum, thioglycolate liquid culture medium test tube bacteria liquid turbid, microscopic examination for gram-positive bacilli
Conclusion	good viability, no abnormal colony morphology, qualified

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