Streptococcus agalactiae|242996 |BNCC

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Streptococcus agalactiae Lehmann and Neumann

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ° C; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, easy to pick up, G+(blue purple), cocci
Sharing mode	Public welfare sharing

Streptococcus agalactiae Lehmann and Neumann

1. Description

1. Name: Streptococcus agalactiae Lehmann and Neumann

2. BNCC No.: 242996

3. Biosafety level: 3

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Brain Heart Infusion Medium (BHI): Brain Infusion Powder 5.0g, Heart Infusion Powder 12.5g, Peptone 5.0g, Tryptone 10.0g, Sodium Chloride 5.0g, Glucose 2.0g, Disodium Hydrogen Phosphate 2.5g, Agar 20.0 g (not included in liquid medium), pH7.4±0.2. Sterilize at 121°C for 15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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