Green Trichoderma|341743 |BNCC

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Trichoderma viride

Subculture procedure	①Prepare one 90mm solid agar plate, one inoculation shovel, and one inoculation hoe for typical bacterial colonies; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious white colonies and dense hyphae on the comprehensive PDA medium, spreading and growing towards the edge of the Agar plate. The back of the medium is light yellow
Sharing mode	Public welfare sharing

Trichoderma viride

Storage conditions: 2~8 ℃

No. 341743

Product format: agar slant in 14mm test tube

Validity period : growing culture, in 30 dyas

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, integrated PDA,3-5 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4) lay flat the small pieces to the center of the agar plate;
(5)put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 4 days plate colonies are obvious
colony morphology:	small filamentous fungi, with obvious colonies on the integrated PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, and light yellow on the back of the medium
Conclusion:	good viability, no abnormal colony morphology, qualified

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