Brucella sluggish|189797 |BNCC

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Lentilactobacillus buchneri

Culture medium	BNCC370433
Description	de Man, Rogosa and Sharpe (MRS) Broth
Composition	Peptone:10.0g, Beef extract:8.0g, Yeast powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, (NH₄)₂HC₆H₅O₇:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, pH:5.7±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing