Black carbon Aspergillus|193598 |BNCC

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Aspergillus carbonarius

Subculture procedure	① Prepare the above two tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, producing a large number of spores, producing black spores, with a yellow front, lighter color, rough surface, and dull surface
Sharing mode	Public welfare sharing

Aspergillus carbonarius

BNCC No.：193598

Biosafety level：4

Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. Synthesis PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling liquid:200g potato, peel, cut into pieces, boil in distilled water 30min, take the filtrate to 1000mL, for later use. 121 ℃,15min.

2. Atmosphere： aerobic

3. Temperature：25-28℃

Notes：
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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