Schenker Sporothrix NIH 7019|264969 |BNCC

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Sporothrix schenckii Hektoen et Perkins

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃, 5-7 days, aerobic
Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	2-8 ° C
Safety level	2
morphology	The diameter of the colony is 1-3mm, small in size, white in color, with a slightly fuzzy surface. After maturity, the surface is fuzzy, with a raised center and irregular edges. The back shows dark pigment diffusion
Sharing mode	Public welfare sharing