Prevotella aeruginosa|359116 |BNCC

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Prevotella histicola Downes et al. 2008

Subculture procedure	①Draw a line on the Agar plate and prepare 2 plates (equilibrate in anaerobic environment for 24 hours) with typical bacterial colonies; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 48-72h; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with well-defined margins. Superficial appearance: grayish-white, convex in the center, smooth surface, glossy, moist texture, easily lifted. Gram-negative (red), bacillus. Purity: Pure.
Sharing mode	Public welfare sharing

Prevotella histicola

Storage conditions : 2~8 ℃

No. : 359116

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be deemed as good receipt. Dry powder shall not be retained after one use, and it can be used after being activated for 1~2 generations. please refer to the attached page for the use of the flat panel. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),24-48h.

Recovery steps:

① Prepare 2 of the above plates;

② After disinfecting the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck about 0.5mL of sterile water (placed in anaerobic environment for 24 hours) and drive it into a freeze-drying tube. after fully dissolving the bacterial powder, drive it into 2 plates, 200μL/piece, evenly coated;

④ Place the plate under the above culture conditions for 24-48h.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 48hours strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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