Fusarium wilt specialization|226606 |BNCC

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Fusarium oxysporum f. sp. vasinfectum

Subculture procedure	Small filamentous fungi have obvious white colonies and dense hyphae on the comprehensive PDA medium, spreading and growing towards the edge of the Agar plate. The back of the medium is light yellow
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	-196
Safety level	1
morphology	The isolate was a small filamentous fungus. On general PDA medium, it formed distinct, white colonies with dense and vigorous mycelia, exhibiting spreading growth toward the Agar plate periphery. The reverse side of the medium showed a pale yellow coloration.
Sharing mode	Public welfare sharing

1.Description

1. Name： Fusarium oxysporum f. sp. vasinfectum

2. BNCC No.：226606

3. Biosafety level： 4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use.

2. Atmosphere：aerobic

3. Temperature：24-28 ℃

4.Notes：

1.Normal culturing time: 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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