Neisseria gonorrhoeae|270346 |BNCC

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Neisseria gonorrhoeae (Zopf) Trevisan

Subculture procedure	① Dissolve the Frozen vial in a water bath (37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 24-48 hours; 5% CO2
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), cocci
Sharing mode	Public welfare sharing

Neisseria gonorrhoeae

Storage conditions: 2~8 ℃

No. 270346

Product format: 90mm flat plate

Validity period: growing culture, in 10 days

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃,5% CO₂, chocolate plate (ready to use),18-24h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 20h, plate bacteria layer obvious; colony is typical on the streaked plate
colony morphology: (above)	Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: flat surface: bright and smooth texture: moist and viscous
Conclusion	good viability， no abnormal colony morphology, qualified

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