Monascus|336199 |BNCC

Essential Information Certificate Related Products

Monascus purpureus

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	A small filamentous fungus that spreads and grows on PDA medium, with obvious colonies and red hyphae. The hyphae are vigorously spreading and growing, producing red spores
Sharing mode	Public welfare sharing

Monascus purpureus

Storage conditions : 2~8 ℃

No. : 336199

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level :1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :28 ℃, aerobic, PDA,5-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (without liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare a test tube containing 5-10ml sterile water and two agar plates；

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of recovery are reported as follows:

item	test results
viability	good viability, in 5-7 days strain layer is obvious
colony morphology	small filamentous fungi have obvious colonies, red hyphae, vigorous spread and red spores on PDA medium.
conclusion	good viability, no abnormal colony morphology, qualified & nbsp;

Download certificate