Porphyromonas gingivalis|236547 |BNCC

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Porphyromonas gingivalis (Coykendall et al.) Shah and Collins

Subculture procedure	① Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube, thoroughly dissolve and mix; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 8-10 days; anaerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, producing black pigment, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Porphyromonas gingivalis

Storage conditions: 2~8 ℃

No. 236547

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),8-10 days.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates（place in anaerobic environment for 24hours before usage）；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water（place in anaerobic environment for 24 hours before usage） into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 8-10 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 10 days strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Irregular Edge: Irregular Transparency: Opacity color: gray-black bulge: middle bulge surface: bright and smooth texture: viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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