Lactobacillus delbrueckii subsp. bulgaricus|137361 |BNCC

Essential Information Certificate Related Products

Lactobacillus delbrueckii subsp. bulgaricus

Subculture procedure	① Prepare 1-2 fresh plates mentioned above (placed in an anaerobic environment for deoxygenation for 24 hours in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium or sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. Dissolve it thoroughly and mix well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 24-48 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	G+(blue purple), Bacillus, purity: pure, bacterial solution turbid with white precipitate
Sharing mode	Public welfare sharing

Lactobacillus delbrueckii subsp.

Storage conditions: 2~8 ℃

No.: 137361

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, MRS medium, 48-72h. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7 & plusmn;0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a φ18mm test tube of 10ml liquid medium;

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4)Put the test tube under the above culture conditions for cultivation. The culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 48-72h a large number of bacteria precipitate at the bottom of the liquid medium, and the plate strain does not grow.
Colony morphology: (pictured above)	Lactobacillus delkii subsp. Bulgaria, a gram-positive bacterium, the bottom of MRS liquid culture is turbid, the plate strain does not grow, build discuss the use of liquid culture.
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate