Botrytis cinerea (tomato early blight pathogen)|337910 |BNCC

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Alternaria solani

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	Liquid nitrogen
Safety level	1
morphology	The initial hyphae are white, but later turn grayish black. The colony is fuzzy and thick, with a black center on the back and yellow edges
Sharing mode	Public welfare sharing

Alternaria solani

Storage conditions: 2~8 ℃

No.: 337910

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates;
(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, 5-7 days.Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 5 days strain layer is obvious
colony morphology	small filamentous fungi, colonies are obviously visible on integrated PDA culture medium, hyphae are gray-white to black, hyphae are dense and thick, grow rapidly, and the back is black.
conclusion	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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