Fusarium graminearum|342051 |BNCC

Essential Information References Certificate Related Products

Fusarium graminearum

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake it quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi with obvious colonies on PDA medium, white cotton like hyphae, dense and vigorous, spreading and growing towards the edge of the Agar plate, and light yellow on the back of the medium
Sharing mode	Public welfare sharing

Description

1, Name: Gibberella zeae (Schwein.) Petch

2. BNCC No.: 342051

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural, 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 28 ℃,5-7 days

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate