Penicillium|341625 |BNCC

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Penicillium sp.

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, producing gray brown spores, gray white on the front and golden yellow on the back, purity: pure
Sharing mode	Public welfare sharing

Penicillium sp.

No. : 341625

Storage conditions : 2~8 ℃

Product format : freeze dried, 200ul

Validity period : 6 years

Biosafety level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :28 ℃, aerobic, comprehensive potato medium, 5-7 days, comprehensive potato medium: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g, MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5)Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 5-7 days strain layer is obvious and colony is typical
colony morphology: (above)	filamentous fungi have obvious colonies on comprehensive PDA medium, white hyphae, dense/low-flat hyphae, vigorous spread and brown spores.
conclusion	good viability, no abnormal colony morphology, qualified

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