Erwinia salsa|137733 |BNCC

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Erwinia pyrifoliae

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic (or TSA medium)
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 1–2 mm in diameter, circular, entire, opaque, grayish-white, convex, smooth, shiny, and moist. They are easily picked up and produce no soluble pigment. Microscopic examination reveals Gram-negative rods. The culture appears pure.
Sharing mode	Public welfare sharing