Mycobacterium hominis|365102 |BNCC

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Mycobacterium hominis-BNCC
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  • Mycobacterium simiae-BNCC

Mycobacterium simiae

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  • number：365102
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    The colonies measure 0.5-1 mm in diameter and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth in texture, making them easy to lift. The organisms are acid‑fast stain positive. Purity: pure.
Standard strain Quantitative strain DNA extraction
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Mycobacterium simiae
Subculture procedure ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. During the cultivation process, the agar plate should be kept moist. After the agar plate is dry, it can be opened in a safe cabinet and an appropriate amount of liquid medium containing additives should be added. The colony diameter is 0.5-1mm, opaque, yellow on the front, light in color, protruding in the middle, smooth on the surface, easy to pick up, and positive for acid resistant staining,
Growth conditions 37 ℃; 5-7 days; aerobic;
Storage conditions 2-8 ℃
Safety level 2
morphology The colonies measure 0.5-1 mm in diameter and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth in texture, making them easy to lift. The organisms are acid‑fast stain positive. Purity: pure.
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