Mycobacterium hominis|365102 |BNCC

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Mycobacterium simiae

Culture medium	BNCC360851
Description	Middlebrook 7H10 Agar
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.5g, Na₂HPO₄:1.5g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.025g, CaCl₂:0.5mg, ZnSO₄:0.001g, CuSO₄:0.001g, Sodium L-Glutamate(C₅H₈NNaO₄):0.5g, C₆H₈FeNO₇:0.04g, Pyridoxine Hydrochloride(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions	37 ℃; 5-7 days; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. During the cultivation process, the agar plate should be kept moist. After the agar plate is dry, it can be opened in a safe cabinet and an appropriate amount of liquid medium containing additives should be added. The colony diameter is 0.5-1mm, opaque, yellow on the front, light in color, protruding in the middle, smooth on the surface, easy to pick up, and positive for acid resistant staining,
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 0.5-1 mm in diameter and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth in texture, making them easy to lift. The organisms are acid‑fast stain positive. Purity: pure.
Sharing mode	Public welfare sharing