Helicobacter pylori|372308 |BNCC

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Helicobacter pylori (Marshall et al.) Goodwin et al.

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 3-4 days; 5% CO2
Subculture procedure	①Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Dissolve the frozen storage tube in a 37 ℃ water bath, shake quickly to dissolve, wipe the outer wall of the frozen storage tube with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, pour it into 1-2 agar plates, and apply evenly; ④ Cover the agar surface with sufficient (1-4ml) tryptone soy broth medium to maintain a liquid film throughout the entire cultivation period, and prevent the culture from growing on dry surfaces. ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, semi transparent, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Vibrio, purity: pure
Sharing mode	Public welfare sharing