Clostridium perfringens|373850 |BNCC

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Culture medium	BNCC372404
Description	Reinforced Clostridial Medium (RCM)
Composition	Yeast Extract:3.0g, Beef Infusion Powder:10.0g, Glucose:5.0g, Pancreatic Digest of Casein:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CH₃COONa:3.0g, Agar:0.5g, pH:6.8±0.1(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic
Subculture procedure	① Prepare one 18mm test tube liquid medium, about 10mL (placed in anaerobic environment for deoxygenation for 24 hours in advance); ② Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer the bacterial solution to a new liquid culture medium using a pipette or suction tube in an anaerobic environment; ④ Incubate the liquid test tube under the above cultivation conditions until the bacterial solution becomes turbid and ready for use.
Storage conditions	-80 ℃
Safety level	1
morphology	Gram‑positive (G⁺, appearing blue‑violet), rod‑shaped (bacillus).
Sharing mode	Public welfare sharing