Huo's ciliates|370224 |BNCC

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Leptotrichia hofstadii

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ° C; 3-7 days; Strictly anaerobic
Subculture procedure	① Dissolve the Frozen vial in a water bath at 37 ℃ and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colonies measure 0.5-1 mm in diameter, are circular with irregular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a raised center, and are glossy, smooth, and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing