Aspergillus niger|360950 |BNCC

Essential Information Certificate Related Products

Aspergillus niger

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, produce a large number of spores, produce black spores, opaque, with light yellow hyphae. After spore production, the front is black, flat in shape, rough on the surface, dark on the surface, dry in texture, purity: pure
Sharing mode	Public welfare sharing

Aspergillus niger

Storage conditions: 2~8 ℃

No. 360950

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:28℃, aerobic, comprehensive PDA, 3-5 days, comprehensive PDA: potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open in the the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 5-7days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 5 days, plate colonies are obvious
Colony morphology: (pictured above)	small filamentous fungi, the colony is obvious on the integrated PDA medium, the initial hyphae are white, dense and vigorous, and the black spores are full of plates.
conclusion: good viability, no abnormal colony morphology, qualified

Download certificate