Ushuaia Sea Pseudomonas|139506 |BNCC

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Marinomonas ushuaiensis

Culture medium	BNCC353703
Description	2216E agar
Composition	Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC₆H₅O₇):0.1g, KBr:0.08g, NaCl:19.45g, MgCl₂:5.98g, Na₂SO₄:3.24g, CaCl₂:1.8g, KCl:0.55g, Na₂CO₃:0.16g, Na₂NO₃:0.0016g, Na₂HPO₄:0.008g, SrCl₂:0.034g, H₃BO₃:0.022g, Na₂SiO₃:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃)
Growth conditions	22-25 ℃; 24-48 hours; aerobic
Subculture procedure	① Dissolve the Frozen vial in a water bath at 30 ℃ and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colonies are 1–2 mm in diameter, circular, entire, convex, opaque, and grayish-white. They have a smooth, shiny, moist surface and are easily picked up. Gram staining reveals Gram-negative rods. The culture is pure.
Sharing mode	Public welfare sharing