Lactobacillus acidophilus|133035 |BNCC

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Lactobacillus acidophilus

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	30 ℃; 48-72h; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2 mm, circular with irregular edges, opaque. The obverse side is white, raised centrally, with a smooth surface and moist texture. Gram-positive (bluish-purple), rod-shaped bacteria, purity: pure.
Sharing mode	Public welfare sharing

Lactobacillus acidophilus

Storage conditions: 2~8 ℃

No. : 133035

Product format : freeze dried,200ul

Validity period : freeze-dried tube 24 months

Biosafety level : 1,handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :37°C, strictly anaerobic, MRS medium. MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2. 121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned MRS plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 40h MRS plate colony is obvious
colony morphology	size: general color: milky white shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: bulge
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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