Mycobacterium smegmatis|364239 |BNCC

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Mycobacterium smegmatis

Culture medium	BNCC360851
Description	Middlebrook 7H10 Agar
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.5g, Na₂HPO₄:1.5g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.025g, CaCl₂:0.5mg, ZnSO₄:0.001g, CuSO₄:0.001g, Sodium L-Glutamate(C₅H₈NNaO₄):0.5g, C₆H₈FeNO₇:0.04g, Pyridoxine Hydrochloride(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions	37 ℃; 3-7 days; aerobic;
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing