Lactobacillus plantarum|134285 |BNCC

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Lactiplantibacillus plantarum

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	37 ℃, 24-48 hours, slightly aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2 mm, circular in shape with regular edges, and opaque. The obverse side is creamy white, centrally raised with a smooth and glossy surface. The texture is moist and easily picked off. Gram-positive (bluish-purple), rod-shaped bacteria, purity: pure.
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Lactobacillus plantarum

Storage conditions : 2~8 ℃

No.: 134285

Product format: freeze dried,200ul

Validity period : freeze-dried tube 24 months

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculter:37°C, anaerobic, MRS medium. MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2. 121 ℃, 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned MRS plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability,in 40h MRS plate colony is obvious
colony morphology:	size: general color: milky white shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: bulge
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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