Porcine Roseberylla|384251 |BNCC

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Roseburia porci

Culture medium	BNCC376181
Description	Modified YCFA Medium Base
Composition	Trypticase:10.0g, Yeast extract:2.5g, Glucose:5g, K₂HPO₄:0.45g, KH₂PO₄:0.45g, NaCl:0.9g, MgSO₄.7H₂O:45.00mg, CaCl₂.2H₂O:90.00mg, Resazurin:1.0mg, Volatile fatty acids:10ml, NaHCO₃:4.0g, Cysteine hydrochloride(C₃H₇NO₂S.HCl):1.0g, Hemin(C₃₄H₃₂ClFeN₄O₄):10.0mg, Vitamin solution:10ml
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Prepare one liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Cloudy liquid culture medium
Sharing mode	Public welfare sharing