Enterococcus faecalis|134272 |BNCC

Essential Information References Certificate Related Products

Enterococcus hirae

Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	37°C, 18-24h, anaerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat edges; opaque, grayish-white to dark gray upper surface; centrally raised; smooth, glossy surface; moist texture; easily liftable; non-pigmented; Gram-positive (blue-violet); cocci; Purity: Pure
Sharing mode	Public welfare sharing

Enterococcus hirae

Storage conditions: 2~8 ℃

No.: 134272

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37 ℃, strictly anaerobic, Columbia blood plate (ready-to-use type),48-72h.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 48-72 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 72h strain layer become obvious, colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm; Shape: round ; Edge: neat ; Transparency: opaque color: white ; Protuberance: middle bulge ; Surface: bright and smooth ; Texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate