Salmonella enterica subsp. enterica|139798 |BNCC

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Salmonella enterica subsp. enterica

Culture medium	BNCC352232
Description	Tryptic Soy Broth
Composition	Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K₂HPO₄:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing