Paracremonium binnewijzendii|144583 |BNCC

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Paracremonium binnewijzendii

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the plate and onto the surface of the agar; ⑤ Cultivate the flat plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	30 ℃; 7-10 days; Aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on PDA medium, pink white, raised colonies, and golden yellow on the back of the medium
Sharing mode	Public welfare sharing

Paracremonium binnewijzendii

Storage conditions: 2~8 ℃

No. 144583

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, PDA,7-10 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (without liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 3-5 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 10days strain layer become obvious
colony morphology:	small filamentous fungi, with obvious colonies on PDA medium, pink white, raised colonies, and golden yellow on the back of the medium.
Conclusion:	good viability, no abnormal colony morphology, qualified

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