Orange Penicillium|146100 |BNCC

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Penicillium citrinum

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate all liquid test tubes and plates under the above culture conditions, and the strains can be used once they grow. Small filamentous fungi have obvious colonies on Czapek's agar medium, with white initial hyphae, dense and vigorous growth, covering the plates, and yellow on the back
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	A small filamentous fungus. Colonies are conspicuous on Czapek's agar medium, with mycelia initially white, dense and vigorous, covering the entire Agar plate; yellow pigmentation is observed on the reverse side.
Sharing mode	Public welfare sharing

Penicillium citrinum

Storage conditions: 2~8 ℃

No. 146100

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28℃, aerobic, Czapek's agar, 5-7 days, Czapek's agar: sucrose 30.0 g, NaNO3 3.0 g, MgSO4 7H2O 0.5 g, KCl 0.5 g, FeSO4 4H2O 0.01 g, K2HPO4 1.0 g, agar 15.0 g, distilled water 1.0 L, pH 6.0-6.5

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 5 days plate colony is obvious
colony morphology:	small filamentous fungus, with obvious colonies on Czapek ' sagar medium, with white initial hyphae, Dense and exuberant, full of plates, yellow on the back
Conclusion:	good viability, no abnormal colony morphology, qualified

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