Numerous fungi|148756 |BNCC

Essential Information References Certificate Related Products

Aspergillus creber

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread growth, filamentous, white on the front, yellow on the back, raised in the middle, purity: pure
Sharing mode	Public welfare sharing

Aspergillus glaucus

Storage conditions : 2~8 ℃

No. : 148756

Product format :agar slant in 14mm test tube

Validity : growing culture, in 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, comprehensive PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 *7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

① Prepare 1-2 integrated PDA tablets;

②After disinfection of the inclined surface of the test tube, open it in the safety cabinet;

③ cut into pieces with sterile inoculation hoe and inoculation shovel (see attached page),0.5×0.5cm2 small square block;

④ Place the small square block flat on the center of the plate and on the agar surface;

⑤ The plate is being cultured under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 7days strain layer become obvious
colony morphology:	small filamentous fungi have obvious colonies on comprehensive PDA medium, with white hyphae at the initial stage, dense and low flat, and gray-green hyphae at the later stage, with ring lines and gray-green spores.
Conclusion:	good viability, no abnormal colony morphology, qualified

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