BeNa Culture Collection
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| Culture medium | BNCC370527 |
| Description | Yeast Mold (YM) Medium |
| Composition | Yeast extract:3.0g, Malt extract:3.0g, Glucose:10.0g, Casein peptone:5.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 28 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, milky white on the front, raised in the middle, smooth on the surface, viscous in texture, easy to pick up, G+(blue purple), cocci (oval shaped), purity: pure |
| Sharing mode | Public welfare sharing |
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