BeNa Culture Collection
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| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Growth conditions | 28 ℃; 2-3 days; aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Colony diameter: 1–2 mm, circular, regular margin, opaque, pale red on the obverse, convex in the center, smooth and shiny surface, moist texture, easily picked, Gram‑positive (bluish‑purple), rod‑shaped, purity: pure. |
| Sharing mode | Public welfare sharing |
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