Aspergillus nidulans|185653 |BNCC

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Aspergillus nidulans

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, produce a large number of spores, produce yellow spores, irregular edges, opaque, yellow front, flat shape, rough surface, wrinkled surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Aspergillus nidulans

Storage conditions: 2~8 ℃

No,: 185653

Product format: freeze dried

Validity period: 24 months

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, integrated PDA. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, glucose 20.0g, vitamin B1 10mg, agar 20g,pH natural. 121 ℃,15min. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 pieces of SDA plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a SDA agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 5 days plate colony is obvious
colony morphology:	The morphology of the bacteria is obviously visible in the integrated PDA agar medium, and the colony edge is white, yellow-green spores are produced, dry and vigorous, and spread to the entire flat surface.
conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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