Blue plow fungi|185750 |BNCC

Essential Information Certificate Related Products

Absidia coerulea

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 2-3 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	A small filamentous fungus. On synthetic PDA medium, the colony is white and cotton‑like, spreading toward the edge of the Agar plate. In later stages, the colony turns gray, while the reverse side of the medium shows a pale yellow coloration.
Sharing mode	Public welfare sharing

Absidia coerulea

Storage conditions: 2~8 ℃

No. 185750

Product format: agar slant in 14mm test tube

Validity: growing culture in 30days

Biosafety y level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, integrated PDA, 2-3 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

① Prepare 1-2 pieces of PDA plates;

② Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

③ Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

④ Lay flat the small pieces to the center of the agar plate;

⑤ Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 3days strain layer become obvious
colony morphology:	small filamentous fungi, the colony on the integrated PDA medium is white, cotton wool-like, spreading to the edge of the plate, the colony is gray at the later stage, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate