Streptococcus agalactiae|185941 |BNCC

Essential Information References Certificate Related Products

Streptococcus agalactiae

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	Colony diameter: 0.5–1 mm, circular, regular margin, opaque, grayish-white on the obverse and yellow on the reverse, convex in the center, smooth and shiny surface, moist texture, easily picked, non‑pigmented, Gram‑positive (bluish‑purple), coccus, purity: pure.
Sharing mode	Public welfare sharing

Streptococcus agalactiae

No. 185941

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 2 , handle in safety cabinet

Storage conditions: 2~8 ℃

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, BHI: 4.0g of bovine brain extract powder, 4.0g of bovine heart extract powder, 5.0g of peptone, 16.0g of casin peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, pH7.4 ± 0.2. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of BHI liquid media or two BHI agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ ; or directly dispense 200ul of the liquid suspension into a BHI agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 24h BHI bacterial liquid is turbid, BHI strain layer is obvious
colony morphology: (above)	Size: Smaller Color: White Shape: Round Edge: Neat Edge Surface: Smooth and Bright Transparency: Opaque Texture: Slightly Dry
Conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

Download certificate