Listeria monocytogenes|185989 |BNCC

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Listeria ivanovii

Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light yellow on the front, raised in the middle, smooth and bright on the surface, moist texture, no pigment production, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1. Description

1. Name: Listeria ivanovii

2. BNCC No.: 185989

3. Biosafety level: 3

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Brain heart extract agar (BHI): heart extract 5.0g, brain extract 12.5g, monthly peptone 10.0g, glucose 2.0g,NaCl 5.0g,Na2HPO4 2.5g, agar 15.0g, distilled water 1.0L,pH7.4.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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