Aspergillus niger|187647 |BNCC

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Aspergillus niger

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	It exhibits spreading growth on complex potato agar plates. The surface appears white and fluffy, eventually producing black spores. The reverse side is yellowish-brown with radial furrows.
Sharing mode	Public welfare sharing

Aspergillus niger

Storage conditions: 2~8 ℃

No. 187647

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28℃, aerobic, comprehensive PDA, 5-7 days, comprehensive PDA: potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L

Recovery steps:

① Prepare the above flat plate 1-2 block;

②After disinfecting the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck 0.5mL of sterile water and put it into the freeze-drying tube. after fully dissolving the bacterial powder, put it into the plate 200 & mu;L/piece, coated evenly;

④ Place the plate under the above culture conditions for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 5-7 days plate colony is obvious
colony morphology:	small filamentous fungi, with obvious colonies on comprehensive PDA medium, The hyphae gradually changed from white to yellow, and later produced black spores and covered with plates.
Conclusion:	good viability, no abnormal colony morphology, qualified

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